Genotype screening plays a pivotal role in the prevention of hereditary disorders, notably sickle cell disease, particularly among adolescents. Early identification of genetic status facilitates informed health, reproductive, and marital decisions. This study investigated the level of knowledge and uptake of genotype screening among Undergraduate Students of Lagos State University of education. A descriptive crosssectional study design was employed, utilizing a structured, researcher-developed questionnaire divided into five sections. Data were collected from 149 undergraduate students and analyzed using SPSS version 26. Results were presented using descriptive statistics, including tables, frequencies, and percentages. Findings indicated that 81.2% of respondents (n=121) were aware of genotype screening, with the school identified as the main source of information. However, 65.1% (n=97) erroneously equated genotype screening with blood group testing, reflecting notable misconceptions. The uptake of genotype screening, as reported by 77.2% of respondents, was largely influenced by factors such as family support, peer influence, and access to healthcare services. Chi-square analysis revealed no statistically significant association between age and knowledge level (p > 0.05), nor between awareness and familiarity with genotype screening (p > 0.05). The study recommends the integration of genotype education into the university curriculum. In addition, collaborative efforts among healthcare professionals, educators, and parents are essential to dispel misconceptions and enhance the uptake of genotype screening among the youths.