This study investigates the sub-lethal effects of herbicide exposure on Channa punctata (Bloch), a commonly found freshwater teleost, through an integrated approach involving histopathological, hematological, and biochemical assessments. Given the increasing prevalence of herbicide contamination in aquatic ecosystems due to agricultural runoff, it is essential to understand its physiological impact on nontarget organisms such as fish. Specimens were exposed to a sub-lethal concentration of a commercially available herbicide for a period of 30 days. Histopathological analyses revealed pronounced tissue damage in both gills and liver, including epithelial lifting, lamellar disorganization, hepatic vacuolization, and cellular necrosis, indicating compromised respiratory and detoxification functions. Hematological parameters such as red blood cell count, hemoglobin concentration, and hematocrit levels were significantly reduced, suggesting anemia and impaired oxygen-carrying capacity, while an increase in white blood cell count indicated an immune response to systemic stress. Biochemical alterations, including elevated levels of glucose, ALT, and AST and reduced total protein content, further substantiated the physiological stress and liver dysfunction caused by herbicide exposure. These findings demonstrate that herbicides, even at sub-lethal levels, can induce significant structural and functional impairments in fish, potentially threatening their survival and the ecological balance of aquatic environments. The study underscores the importance of regulating agrochemical use and implementing biomonitoring programs to assess aquatic ecosystem health