Research Problem: "A pre-experimental study to assess the effectiveness of information booklet regarding prevention of hypoglycemia in neonates among postnatal mothers at Government Rajindra Hospital Patiala, Punjab." Aim of the Study: To improve the knowledge regarding prevention of hypoglycemia in neonates among postnatal mothers. Methodology: A quantitative approach with preexperimental research design was used for this study. A sample size of 30 postnatal mothers in Government Rajindra Hospital, Patiala. In this study purposive sampling technique was used. Structured questio naire was developed and admini stered to assess the effectiveness of information booklet regarding prevention of hypoglycemia in neonates. Result: The mean knowledge score ofpretestwas8.67andpost-testwas17.47. There is significant difference in the pre-test and post-test knowledge score regarding prevention of hypoglycemia in neonates among postnatal mothers in Government Rajindra Hospital, Patiala. There is significant association between the levels of knowledge sources of information among postnatal mothers. There is no significant association between the level of knowledge and other demographic of resi dence, family income, and source of information. Conclusion: Out of 30 subjects, in pre-test majority 25(83.3%) had poor knowledge score, 5(16.7%) subjects had average knowledge and the remaining 0(0.00%) had good knowledge score of postnatal mothers regarding prevention of hypoglycemia in neonates. But in the posttest postnatal mothers in Government Rajindra Hospital, Patiala, the most of 26(86.7%) had average knowledge, followed by 4(13.3%) had good knowledge and the remaining 0(0.00%) had poor knowledge. There was no association between demographic variables age / education / occupation area of residence, family income and source of information. The calculated chi-square values were less than the table value at the 0.05 level of significance.